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Reagent Description
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Antibodies
MR4 Number:
MRA-16
Price:
$0.00
Organism:
Plasmodium vivax
Designations:
PvMSP-1/19 rabbit antiserum
Depositors:
JH Adams
Biosafety Level:
1
Shipped:
frozen
Classification:
KINGDOM: Protozoa
Comments:
rabbit antiserum against purified recombinant P. vivax (Sal-1) MSP-1 19 kD fragment [
111544
]
Specificity: MSP-1 protein of Plasmodium vivax
Immunogen: The protein, designated PvMSP1-19/290516 and donated by D.C. Kaslow (MVDU/NIH), was expressed in yeast as a secreted protein, purified and emulsified with adjuvant for immunizations. [
111544
]
Freund's (1 x complete, 3 x incomplete) was used as an adjuvant and the serum was decomplemented. [
111544
]
Source of antibody: rabbit serum [
111544
]
Quality control methods: ELISA, Western blot
Additional information: Serum antibodies reacted with both reduced and nonreduced purified recombinant PvMSP-1 (PvMSP1-19/290516) by western immunoblot. [
111544
]
Serological reactivity measured by indirect ELISA against purified recombinant PvMSP-1 (PvMSP1-19/290516) estimated a titer of >900,000 (1:60,000 at the titration midpoint). [
111544
]
-20C [
111544
]
Plasmodium vivax MSP-1 19 kDa fragment [
111544
]
References:
111544: John H Adams, personal communication
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