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MRA-220  dhfr-M5 Plasmodium falciparum
(Primers and Probes)
Organism: Plasmodium falciparum
Descriptions: dhfr-M5
Depositors: Malaria Research and Reference Reagent Resource Center
Biosafety Level: 1
Shipped: freeze-dried
Comments: Quantity limit per order for this item is 1. This item can be ordered once a year. Orders over this limit will be sent to NIAID for approval before shipment.

Dissolving the contents of each vial (2.5 nmol) in 0.1 ml of distilled water with its partner primer (total volume 0.2 ml) will allow for 200 PCR reactions at 1 microliter per reaction when used at 500 nM.
  Dissolving the contents of each vial (2.5 nmole) in 100µl of distilled water and mixing with its partner primer ( total volume of 200µl), it can be used for 200 PCR reactions (each reaction uses 1 µl in a 25µl volume) at 500 nM. If used at 250 nM, 400 reactions can be run.
If used at 250 nM, 400 reactions can be run.
If three or four primers are used, the above volumes would be 1.5 and 2.0 microliters, respectively, per reaction.
Sequence (5' to 3'): AGT ATA TAC ATC GCT AAC AGA [53406]
Purity: Standard
%GC: 33
Primer Length: 21

This primer is used for the detection of [dihydrofolate reductase] (Plasmodium falciparum)
Store at: -20°C
Availability Status: In Stock
References: 53406: Duraisingh MT, et al. Plasmodium falciparum: detection of polymorphisms in the dihydrofolate reductase and dihydropteroate synthetase genes by PCR and restriction digestion. Exp. Parasitol. 89: 1-8, 1998.
Citations: Acknowledgment for publications should read "The following reagent was obtained through the MR4 as part of the BEI Resources Repository, NIAID, NIH: dhfr-M5, MRA-220."
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MRA-219  
dhfr-M1 Plasmodium falciparum
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BEI Resources products are intended for laboratory research purposes only. They are not intended for use in humans.

While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this site, BEI Resources makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. BEI Resources does not warrant that such information has been confirmed to be accurate.


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