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Wild Stocks
|
| Strain |
Organism |
MRA No. |
Origin |
Notes |
|
|
A. gambiae
|
112
|
Gambia
|
wild-type stock that has been in culture for over 30 years. It is considered a standard research organism, TEP1 s/s, G3 is primarily a Savanna type stock
|
|
|
A. gambiae
|
186
|
Kenya
|
This strain was recently found to have a potential translocation of the X chromosome onto the Y chromosome
|
|
|
A. gambiae
|
763
|
Mali
|
This is a wild-type Mopti rDNA form stock.
|
|
|
A. gambiae
|
762
|
Kenya
|
This strain is considered the insecticide susceptible standard
|
|
|
A. stephensi
|
128
|
India
|
This strain is often used in Plasmodium berghei studies
|
|
|
A. atroparvus
|
493
|
Spain
|
wild type
|
|
|
A. freeborni
|
130
|
United States
|
wild type, wild eye and body color, excellent sporozoite-producer strain
|
|
|
A. farauti
|
489
|
Papua New Guinea
|
Infects well with most Plasmodium species. An. farauti no. 1
|
|
|
A. arabiensis
|
339
|
Zimbabwe
|
This strain is considered a standard research organism
|
|
|
A. arabiensis
|
856
|
Sudan
|
This strain was created for the SIT program in Sudan
|
|
|
A. quadrimaculatus
|
139
|
United States
|
wild type colony. An. quadrimaculatus A
|
|
|
A. albimanus
|
126
|
El Salvador
|
This stock was developed during the SIT program in El Salvador
|
|
|
A. quadriannulatus
|
761
|
South Africa
|
wild type, A. gambiae complex member
|
|
|
A. dirus
|
700
|
Thailand
|
force mated species. A. dirus A
|
|
|
A. minimus
|
729
|
Thailand
|
wild type colony. A. minimus A
|
|
|
A. merus
|
803
|
South Africa
|
wild-type, A. gambiae complex member
|
|
|
A. gambiae
|
861
|
Mali
|
This strain was used in the recent genome project (www.vectorbase.org) and is the Savanna rDNA form standard
|
|
|
A. gambiae
|
860
|
Mali
|
This strain was used in the recent genome project (www.vectorbase.org) and is the Mopti rDNA form standard
|
Insecticide Resistance
|
| Strain |
Organism |
MRA No. |
Insecticide/ Origin |
Notes |
Thumbnail |
|
|
A. gambiae
|
594
|
DDT/ Zanzibar
|
This is a DDT-resistant stock. ZAN/U expresses both a glutathione-S-transferase mechanism and elevated beta-esterase activity
|
|
|
|
A. gambiae
|
334
|
Permethrin/ Kenya
|
This strain expresses both the L1014S KDR mechanism and increased cytochrome P450 activity
|
 |
|
|
A. gambiae
|
698
|
Permethrin/ Kenya
|
This strain was selected from RSP for a standard karyotype. Unlike RSP, this strain only expresses the L1014S KDR mechanism
|
|
|
|
A. gambiae
|
115
|
Dieldrin
|
Dieldrin resistant due to GABA mutation (Ala296Gly) combined with a pericentric inversion on chromosome 2
|
 |
|
|
A. gambiae
|
116
|
Dieldrin
|
Dieldrin resistant due to GABA mutation (Ala296Gly)
|
 |
|
|
A. arabiensis
|
764
|
Dieldrin/ Sudan
|
Dieldrin resistant due to GABA mutation (Ala296Ser)
|
 |
Transgenic
|
| Strain |
Organism |
MRA No. |
Notes |
Thumbnail |
P-BAC-0
|
A. gambiae
|
335
|
eGFP expression particularly in the anterior stomach and distal lobes of salivary glands; Contains pK[BIGalpha] insertion in 28D. See Genbank accession numbers AF402294 and AF402295
|
 |
IV
|
A. stephensi
|
314
|
L4s of this stock showed GFP expression primarily in gastric cecae and the posterior stomach
|
 |
SRtTA
|
A. stephensi
|
728
|
SRtTA is homozygous for Actin 5C-eGFP marker with an autosomal insertion of a reverse tTA which is controlled by a serpin 10 promoter fragment. Progeny crosses to a LacZ reponder line reveal staining in the midgut, hemocytes and pericardial cells of larvae and adults.
|
 |
StTA/ tetOPLacZ
|
A. stephensi
|
727
|
This stock has an X-linked tetracycline transactivator gene driver (tTA1) which is controlled by a seprin 10 promoter fragment. Regulation of this system is done via doxycycline. This strain is double marked with an Actin 5c-eGFP and an Actin 5C-dsRed1 marker. Lines carrying individual insertions should be isolated following outcrossing to wild type A. stephensi.
|
 |
B1
|
A. stephensi
|
855
|
RNA silencing of eGFP transcripts in transgenic A. stephensi mosquitoes. pIR-EGFP insertion with dsRed marker under Actin5C promoter. Parental crosses result in 43% reduction in GFP expression. Backcrosses of progeny to B1 males results in 73% reduction in expression.
|
 |
19.1
|
A. stephensi
|
854
|
RNA silencing of eGFP transcripts in transgenic A. stephensi mosquitoes. pIR-EGFP insertion with dsRed marker under Actin5C promoter. Parental crosses result in a 37% reduction in GFP expression. Backcrosses of progeny to B1 males results in a 65% reduction in expression.
|
 |
Encapsulation Effect
|
| Strain |
Organism |
MRA No. |
Notes
|
Thumbnail |
|
|
A. gambiae
|
114
|
This strain has the unique property of being selected for a hyper-reactive immune reaction to infection with Plasmodium cynomolgi B. When infected, >95% of oocysts will be encapsulated within the midgut when compared to 4ARR (MRA-121). This stock is also fixed for the TEP1 allele which may confer resistance to P. berghei infection
|
 |
|
|
A. gambiae
|
121
|
This strain has the unique property of being selected for a lack of immune reaction to Plasmodium cynomolgi B infection. When infected, >95% of oocysts in the midgut are not encapsulated when compared to L3-5 (MRA-114). 4ARR also has a prominent pumpkin eye color mutation marker
|
 |